![]() Undifferentiated cells retained expression of pluripotency markers (OCT4, NANOG, SSEA-4, TRA-1-60 and TRA-1-81), a stable karyotype during long-term culture and could be transfected efficiently with plasmid DNA and short interfering RNA. Both lines were successfully maintained as colonies by mechanical passaging on mouse embryonic feeder cells or as monolayers by trypsin-passaging in feeder-free conditions on Matrigel. Here, we describe derivation of hESC lines, NOTT1 and NOTT2, from human blastocysts graded as 3BC and 3CB, respectively. The ability to maintain human embryonic stem cells (hESCs) during long-term culture and yet induce differentiation to multiple lineages potentially provides a novel approach to address various biomedical problems. Priddle, Helen Allegrucci, Cinzia Burridge, Paul Munoz, Maria Smith, Nigel M Devlin, Lyndsey Sjoblom, Cecilia Chamberlain, Sarah Watson, Sue Young, Lorraine E Denning, Chris Derivation and characterisation of the human embryonic stem cell lines, NOTT1 and NOTT2.
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